Cytochemical characterization of pituitary target cells for biotinylated gonadotropin releasing hormone.

TitleCytochemical characterization of pituitary target cells for biotinylated gonadotropin releasing hormone.
Publication TypeJournal Article
Year of Publication1983
JournalPeptides
Volume4
Issue4
Pagination549-55
Date Published1983 Jul-Aug
ISSN0196-9781
AbstractThese studies describe the application of new cytochemical stains that co-localize a biotin-labeled gonadotropin releasing hormone (GnRH) analog and FSH or LH in the same field or cell. Pituitary monolayer cells were stimulated with the [D-Lys6] GnRH analog or the same analog labeled with biotin. Biotinylated [D-Lys6] GnRH exhibited a higher affinity and was 7-10 X more potent than unlabeled [D-Lys6] GnRH. The avidin-biotin peroxidase complex technique (ABC) was applied to localize the biotinylated GnRH on the cells with the use of a dense black peroxidase substrate. Specificity tests showed that the stain could be eliminated by competition with unlabeled [D-Lys6] GnRH. The GnRH stain was followed by immunocytochemical stains for LH beta, FSH beta or 25-39ACTH with a different peroxidase substrate (amber or orange-red). Stain for GnRH was found on the surfaces of 16% of the cells and 60-90% of the GnRH stained cells also stained for one of the gonadotropins. Most (90-100%) of the gonadotropes showed stain for GnRH. Our studies demonstrate that a potent biotinylated GnRH analog binds cells that can be identified specifically as gonadotropes.
URLhttps://linkinghub.elsevier.com/retrieve/pii/0196-9781(83)90061-X
PubMed Linkhttp://www.ncbi.nlm.nih.gov/pubmed/6316297?dopt=Abstract
Short TitlePeptides