Endobrevin/VAMP-8 is the primary v-SNARE for the platelet release reaction.

TitleEndobrevin/VAMP-8 is the primary v-SNARE for the platelet release reaction.
Publication TypeJournal Article
Year of Publication2007
JournalMolecular biology of the cell
Volume18
Issue1
Pagination24-33
Date Published2007 Jan
ISSN1059-1524
AbstractPlatelet secretion is critical to hemostasis. Release of granular cargo is mediated by soluble NSF attachment protein receptors (SNAREs), but despite consensus on t-SNAREs usage, it is unclear which Vesicle Associated Membrane Protein (VAMPs: synaptobrevin/VAMP-2, cellubrevin/VAMP-3, TI-VAMP/VAMP-7, and endobrevin/VAMP-8) is required. We demonstrate that VAMP-8 is required for release from dense core granules, alpha granules, and lysosomes. Platelets from VAMP-8-/- mice have a significant defect in agonist-induced secretion, though signaling, morphology, and cargo levels appear normal. In contrast, VAMP-2+/-, VAMP-3-/-, and VAMP-2+/-/VAMP-3-/- platelets showed no defect. Consistently, tetanus toxin had no effect on secretion from permeabilized mouse VAMP-3-/- platelets or human platelets, despite cleavage of VAMP-2 and/or -3. Tetanus toxin does block the residual release from permeabilized VAMP-8-/- platelets, suggesting a secondary role for VAMP-2 and/or -3. These data imply a ranked redundancy of v-SNARE usage in platelets and suggest that VAMP-8-/- mice will be a useful in vivo model to study platelet exocytosis in hemostasis and vascular inflammation.
URLhttps://www.molbiolcell.org/doi/full/10.1091/mbc.e06-09-0785?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%3dpubmed
DOI10.1091/mbc.e06-09-0785
PubMed Linkhttp://www.ncbi.nlm.nih.gov/pubmed/17065550?dopt=Abstract
Short TitleMol Biol Cell