Methods for the large-scale synthesis of psoralen furan-side monoadducts and diadducts.

TitleMethods for the large-scale synthesis of psoralen furan-side monoadducts and diadducts.
Publication TypeJournal Article
Year of Publication1992
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue10
Pagination4514-8
Date Published1992 May 15
ISSN0027-8424
AbstractWe report methods for the preparation of multimicromole quantities of extremely pure uniquely photo-adducted psoralen-DNA furan-side monoadducts and diadducts (cross-links). The methods use high-intensity krypton and argon ion lasers in the photoreactions and HPLC methods to purify the required oligonucleotides containing the photoadducts. With these methods we have synthesized 2-3 mumol of 8-mer psoralen furan-side monoadduct and diadduct. These methods allow one to generate large amounts of psoralenated DNA oligonucleotides and facilitate their study by NMR and x-ray crystallography.
URLhttp://www.pnas.org/cgi/pmidlookup?view=long&pmid=1584785
PubMed Linkhttp://www.ncbi.nlm.nih.gov/pubmed/1584785?dopt=Abstract
Short TitleProc Natl Acad Sci U S A