Stefan Stamm | College of Medicine

Stefan Stamm

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stefan.stamm@uky.edu

859-323-0896

BBSRB 163

http://www.stamms-lab.net

Position(s):

Professor

Affiliation(s):

Molecular & Cellular Biochemistry

Other Affiliation(s):

MD/PhD Program Internal Advisory Committee

MD/PhD Program Mentor

Research Description:

In higher eukaryotes, genes are interrupted by intervening sequences (introns). After the DNA of a gene is copied into pre-mRNA, these intronic sequences have to be removed and the remaining sequences (exons) are joined.
This process is known as pre-mRNA splicing. Often, a cell can alternatively include or exclude a sequence from the mature mRNA, a process that is known as alternative splicing.

Alternative splicing is an important mechanism to create protein diversity. It allows to create more than one protein from a given pre-mRNA, or to switch off the protein by including a stop codon in its mRNA. Alternative splicing pathways are not static, but subject to changes e.g. in development, or in response to outside stimuli. Numerous examples of alternative splicing have been observed in the brain, where this mechanism might contribute to neuronal plasticity.

See website: http://www.stamms-lab.net/index.html

PubMed Publications:

Welden, JR ; Pawluchin, A ; Doorn, J.; Stamm, S. "Use of Alu Element Containing Minigenes to Analyze Circular RNAs." Journal of visualized experiments : JoVE 157 (2020): [PubMed Link] | [ Full text ]
Welden, JR ; Stamm, S. "Pre-mRNA structures forming circular RNAs." Biochimica et biophysica acta. Gene regulatory mechanisms 1862, 11-12 (2019): 194410. [PubMed Link] | [ Full text ]
Baralle, FE ; Singh, RN ; Stamm, S. "RNA structure and splicing regulation." Biochimica et biophysica acta. Gene regulatory mechanisms 1862, 11-12 (2019): 194448. [PubMed Link] | [ Full text ]
Stamm, S.; Lodmell, JS "C/D box snoRNAs in viral infections: RNA viruses use old dogs for new tricks." Non-coding RNA research 4, 2 (2019): 46-53. [PubMed Link] | [ Full text ]
Luxton, HJ ; Simpson, BS ; Mills, IG ; Brindle, NR ; Ahmed, Z.; Stavrinides, V ; Heavey, S ; Stamm, S.; Whitaker, HC "The Oncogene Metadherin Interacts with the Known Splicing Proteins YTHDC1, Sam68 and T-STAR and Plays a Novel Role in Alternative mRNA Splicing." Cancers 11, 9 (2019): [PubMed Link] | [ Full text ]
Welden, JR ; Zhang, Z.; Duncan, MJ ; Falaleeva, M.; Wells, T.; Stamm, S. "The posterior pituitary expresses the serotonin receptor 2C." Neuroscience letters 684, (2018): 132-139. [PubMed Link] | [ Full text ]
Welden, JR ; Doorn, J.; Nelson, PT ; Stamm, S. "The human MAPT locus generates circular RNAs." Biochimica et biophysica acta. Molecular basis of disease 1864, 9 Pt B (2018): 2753-2760. [PubMed Link] | [ Full text ]
Legendre, M.; Rodriguez-Ballesteros, M.; Rossi, M.; Abadie, V.; Amiel, J.; Revencu, N.; Blanchet, P.; Brioude, F.; Delrue, MA ; Doubaj, Y.; Sefiani, A.; Francannet, C.; Holder-Espinasse, M.; Jouk, PS ; Julia, S.; Melki, J.; Mur, S.; Naudion, S.; Fabre-Teste, J.; Busa, T.; Stamm, S.; Lyonnet, S.; Attie-Bitach, T.; Kitzis, A.; Gilbert-Dussardier, B.; Bilan, F. "CHARGE syndrome: a recurrent hotspot of mutations in CHD7 IVS25 analyzed by bioinformatic tools and minigene assays." European journal of human genetics : EJHG 26, 2 (2018): 287-292. [PubMed Link] |
Stamm, S.; Gruber, SB ; Rabchevsky, AG ; Emeson, RB "The activity of the serotonin receptor 2C is regulated by alternative splicing." Human genetics 136, 9 (2017): 1079-1091. [PubMed Link] | [ Full text ]
Thurman, M.; Doorn, J.; Danzer, B.; Webb, TR ; Stamm, S. "Changes in Alternative Splicing as Pharmacodynamic Markers for Sudemycin D6." Biomarker insights 12, (2017): 1177271917730557. [PubMed Link] | [ Full text ]
Falaleeva, M.; Pages, A.; Matuszek, Z.; Hidmi, S.; Agranat-Tamir, L.; Korotkov, K.; Nevo, Y.; Eyras, E.; Sperling, R.; Stamm, S. "Dual function of C/D box small nucleolar RNAs in rRNA modification and alternative pre-mRNA splicing." Proceedings of the National Academy of Sciences of the United States of America 113, 12 (2016): E1625-34. [PubMed Link] | [ Full text ]
Kwiatkowski, S.; Sviripa, VM ; Zhang, Z.; Wendlandt, AE ; Höbartner, C.; Watt, DS ; Stamm, S. "Synthesis of a norcantharidin-tethered guanosine: Protein phosphatase-1 inhibitors that change alternative splicing." Bioorganic & medicinal chemistry letters 26, 3 (2016): 965-968. [PubMed Link] | [ Full text ]
Zhang, Z.; Shen, M.; Gresch, PJ ; Ghamari-Langroudi, M.; Rabchevsky, AG ; Emeson, RB ; Stamm, S. "Oligonucleotide-induced alternative splicing of serotonin 2C receptor reduces food intake." EMBO molecular medicine 8, 8 (2016): 878-94. [PubMed Link] | [ Full text ]
Matveeva, E.; Maiorano, J.; Zhang, Q.; Eteleeb, AM ; Convertini, P.; Chen, J.; Infantino, V.; Stamm, S.; Wang, J.; Rouchka, EC ; Fondufe-Mittendorf, YN "Involvement of PARP1 in the regulation of alternative splicing." Cell discovery 2, (2016): 15046. [PubMed Link] | [ Full text ]
Falaleeva, M.; Surface, J.; Shen, M.; Grange, P.; Stamm, S. "SNORD116 and SNORD115 change expression of multiple genes and modify each other's activity." Gene 572, 2 (2015): 266-73. [PubMed Link] | [ Full text ]
Jamros, MA ; Aubol, BE ; Keshwani, MM ; Zhang, Z.; Stamm, S.; Adams, JA "Intra-domain Cross-talk Regulates Serine-arginine Protein Kinase 1-dependent Phosphorylation and Splicing Function of Transformer 2β1." The Journal of biological chemistry 290, 28 (2015): 17269-81. [PubMed Link] | [ Full text ]
Shi, Y.; Joyner, AS ; Shadrick, W.; Palacios, G.; Lagisetti, C.; Potter, PM ; Sambucetti, LC ; Stamm, S.; Webb, TR "Pharmacodynamic assays to facilitate preclinical and clinical development of pre-mRNA splicing modulatory drug candidates." Pharmacology research & perspectives 3, 4 (2015): e00158. [PubMed Link] | [ Full text ]
Shen, M.; Falaleeva, M.; Korotkova, N.; Stamm, S. "Direct cloning of double-stranded RNAs." Methods in molecular biology (Clifton, N.J.) 1296, (2015): 53-64. [PubMed Link] | [ Full text ]
Convertini, P.; Shen, M.; Potter, PM ; Palacios, G.; Lagisetti, C.; Grange, P.; Horbinski, C.; Fondufe-Mittendorf, YN ; Webb, TR ; Stamm, S. "Sudemycin E influences alternative splicing and changes chromatin modifications." Nucleic acids research 42, 8 (2014): 4947-61. [PubMed Link] | [ Full text ]
Zhang, Z.; Falaleeva, M.; Agranat-Tamir, L.; Pages, A.; Eyras, E.; Sperling, J.; Sperling, R.; Stamm, S. "The 5' untranslated region of the serotonin receptor 2C pre-mRNA generates miRNAs and is expressed in non-neuronal cells." Experimental brain research 230, 4 (2013): 387-94. [PubMed Link] | [ Full text ]
Falaleeva, M.; Stamm, S. "Processing of snoRNAs as a new source of regulatory non-coding RNAs: snoRNA fragments form a new class of functional RNAs." BioEssays : news and reviews in molecular, cellular and developmental biology 35, 1 (2013): 46-54. [PubMed Link] | [ Full text ]
Kelemen, O.; Convertini, P.; Zhang, Z.; Wen, Y.; Shen, M.; Falaleeva, M.; Stamm, S. "Function of alternative splicing." Gene 514, 1 (2013): 1-30. [PubMed Link] | [ Full text ]
Convertini, P.; Zhang, J.; Grange, P.; Hayward, LJ ; Zhu, H.; Stamm, S. "Genome wide array analysis indicates that an amyotrophic lateral sclerosis mutation of FUS causes an early increase of CAMK2N2 in vitro." Biochimica et biophysica acta 1832, 8 (2013): 1129-35. [PubMed Link] | [ Full text ]
Shen, M.; Bellaousov, S.; Hiller, M.; Grange, P.; Creamer, TP ; Malina, O.; Sperling, R.; Mathews, DH ; Stoilov, P.; Stamm, S. "Pyrvinium pamoate changes alternative splicing of the serotonin receptor 2C by influencing its RNA structure." Nucleic acids research 41, 6 (2013): 3819-32. [PubMed Link] | [ Full text ]
Zhang, Z.; Convertini, P.; Shen, M.; Xu, X.; Lemoine, F.; Grange, P.; Andres, DA ; Stamm, S. "Valproic acid causes proteasomal degradation of DICER and influences miRNA expression." PloS one 8, 12 (2013): e82895. [PubMed Link] | [ Full text ]
Falaleeva, M.; Sulsona, CR ; Zielke, HR ; Currey, KM ; Grange, P.; Aslanzadeh, V.; Driscoll, DJ ; Stamm, S. "Molecular characterization of a patient presumed to have prader-willi syndrome." Clinical medicine insights. Case reports 6, (2013): 79-86. [PubMed Link] | [ Full text ]
Sumanasekera, C.; Kelemen, O.; Beullens, M.; Aubol, BE ; Adams, JA ; Sunkara, M.; Morris, A.; Bollen, M.; Andreadis, A.; Stamm, S. "C6 pyridinium ceramide influences alternative pre-mRNA splicing by inhibiting protein phosphatase-1." Nucleic acids research 40, 9 (2012): 4025-39. [PubMed Link] | [ Full text ]
Pihlajamäki, J.; Lerin, C.; Kaminska, D.; Venesmaa, S.; Itkonen, P.; Boes, T.; Floss, T.; Schroeder, J.; Dearie, F.; Crunkhorn, S.; Burak, F.; Jimenez-Chillaron, JC ; Kuulasmaa, T.; Miettinen, P.; Park, PJ ; Nasser, I.; Zhao, Z.; Zhang, Z.; Xu, Y.; Wurst, W.; Ren, H.; Morris, AJ ; Stamm, S.; Goldfine, AB ; Laakso, M.; Patti, ME "Response to Brosch et al." Cell metabolism 15, 3 (2012): 267-269. [PubMed Link] | [ Full text ]
Zhang, Z.; Kelemen, O.; Santen, MA ; Yelton, SM ; Wendlandt, AE ; Sviripa, VM ; Bollen, M.; Beullens, M.; Urlaub, H.; Lührmann, R.; Watt, DS ; Stamm, S. "Synthesis and characterization of pseudocantharidins, novel phosphatase modulators that promote the inclusion of exon 7 into the SMN (survival of motoneuron) pre-mRNA." The Journal of biological chemistry 286, 12 (2011): 10126-36. [PubMed Link] | [ Full text ]
Shen, M.; Eyras, E.; Wu, J.; Khanna, A.; Josiah, S.; Rederstorff, M.; Zhang, MQ ; Stamm, S. "Direct cloning of double-stranded RNAs from RNase protection analysis reveals processing patterns of C/D box snoRNAs and provides evidence for widespread antisense transcript expression." Nucleic acids research 39, 22 (2011): 9720-30. [PubMed Link] | [ Full text ]
Zhang, Z.; Stamm, S. "Analysis of mutations that influence pre-mRNA splicing." Methods in molecular biology (Clifton, N.J.) 703, (2011): 137-60. [PubMed Link] | [ Full text ]
Khanna, A.; Stamm, S. "Regulation of alternative splicing by short non-coding nuclear RNAs." RNA biology 7, 4 (2010): 480-5. [PubMed Link] | [ Full text ]
Kishore, S.; Khanna, A.; Zhang, Z.; Hui, J.; Balwierz, PJ ; Stefan, M.; Beach, C.; Nicholls, RD ; Zavolan, M.; Stamm, S. "The snoRNA MBII-52 (SNORD 115) is processed into smaller RNAs and regulates alternative splicing." Human molecular genetics 19, 7 (2010): 1153-64. [PubMed Link] | [ Full text ]
Sumanasekera, C.; Watt, DS ; Stamm, S. "Substances that can change alternative splice-site selection." Biochemical Society transactions 36, Pt 3 (2008): 483-90. [PubMed Link] | [ Full text ]
Stamm, S. "Regulation of alternative splicing by reversible protein phosphorylation." The Journal of biological chemistry 283, 3 (2008): 1223-7. [PubMed Link] | [ Full text ]
Kishore, S.; Khanna, A.; Stamm, S. "Rapid generation of splicing reporters with pSpliceExpress." Gene 427, 1-2 (2008): 104-10. [PubMed Link] | [ Full text ]